MGIEasy Exome FS Library Prep Set V2.0 aims to prepare libraries with human gDNA. DNA is fragmented by enzyme digestion, followed by the steps of adaptor ligation at both ends and PCR amplification. The amplified PCR product can be used for exome capture with MGIEasy Exome Capture V4/V5 Probe Set or other commercialized probes.
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Core library preparation kit |
MGIEasy Exome FS Library Prep Kit（16 RXN） |
1000005254 |
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Adaptors |
MGIEasy DNA Adapters-16 (tube) Kit V1.0（16 RXN，16 barcodes） |
1000005284 |
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Clean beads |
MGIEasy DNA Clean Beads |
1000005278 |
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Circularization |
MGIEasy Circularization Kit V2.0 |
1000005260 |
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Other |
MGIEasy Exome Capture Accessory Kit |
1000007743 |
 Compatible with exome probes from various companies The constructed libraries are compatible with probes from Agilent, Nimblegen, IDT, and etc. |  Low DNA input The DNA input can be as low as 200ng, with human gDNA input 50-400ng. |
 Compatible with the automation systems Multiple steps can be finished with the automation systems MGISP-100, such as steps from fragmented DNA to pre-PCR, as well as post-capture elution to post-PCR. |  Quick to prepare libraries and easy to operate Library preparation total time，hands-on time ~30min. |
| Library preparation set | Probes for capture | Compatible sequencing | Read length | Applications |
|---|---|---|---|---|
| MGIEasy Exome Universal Library Prep Set | MGIEasy Exome Capture V4 Probe Set |
BGISEQ-500 DNBSEQ-G400 DNBSEQ-G50 MGISEQ-2000 MGISEQ-200 |
PE100/PE150 |
Whole exome sequencing |
| MGIEasy Exome Capture V5 Probe Set | PE100/PE150 |
Whole exome sequencing |
||
| Other commercialized probes | PE100/PE150 |
Whole exome sequencing/ target sequencing |
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Product name |
MGIEasy Exome FS Library Prep Set |
| Version | V2.0 |
|
Packaging (Part number) |
16 RXN （1000009658） |
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Storage |
-25℃~-18℃，2℃~8℃（Refer to kit label） |
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Required sample input |
50-400ng gDNA；DNA concentration：≥15 ng/μL；intact or mildly degraded DNA；OD260/280=1.8~2.0,OD260/230 >2.0. |
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Sample types |
Tissues, plasma, FFPE samples |
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Compatible species |
Human |
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Library preparation time |
~7 h |
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Hands-on tim |
~30 min |
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Compatible probes |
Probes MGI, Agilent, Nimblegen, IDT |
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Fragmentation methods |
Enzyme digestion (no fragmentation for cfDNA) |
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Library fragment size |
200 -600 bp |
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Compatible sequencing platforms |
BGISEQ-500,MGISEQ-2000, DNBSEQ-G400 |
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Read length |
PE100/PE150 |
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Applications
|
 Library preparation
|
 Sequencing
|
 Bioinformation analysis
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1000009658-MGIEasy Exome FS Library Prep Set User Manual-B0
This file contains comprehensive information about the product, including detailed operating instructions, reagent supplies and equipment to be used.
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